Document Details
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Document Type |
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Thesis |
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Document Title |
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Effect of Artichoke leaves Extract against Gentamicin-Induced Nephrotoxicity in Young Male Rats تأثير مُستخلص أوراق الخرشوف ضد السمية الكلويه التي يحدثها الجنتاميسين في ذكور الجرذان الصغيرة |
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Document Language |
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English |
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Abstract |
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Gentamicin (GM) is an aminoglycoside antibiotic widely used for the treatment of Gram negative bacterial infections. The nephrotoxic effect of GM has limited its clinical use. Several lines of evidence indicate that free radicals are important mediators of GM nephrotoxicity. The present study aimed to evaluate the protective effect of Artichoke leaves extract (ALE) against GM-induced nephrotoxicity in young male rats. Rats were divided into five groups, each of six rats. Group 1 served as negative (-ve) control. Group 2 was injected intraperitoneally (i.p.) with GM (100 mg/kg b.wt/d for 10 days). Groups 3, 4 and 5 were administered orally with ALE with 200,400 or 600 mg/kg b.wt/d, respectively and injected with GM as in group 2. At the end of the experiment, blood samples were collected for serum separation and the kidneys were dissected out for histopathological examination. Phytochemical screening of ALE revealed that, it contains large amounts of flavonoids and saponins. GM injection induced marked nephrotoxicity as evidenced by significant increased in serum levels of kidney function tests, albumin, and K+, with significant decreased in serum levels of total protein and Na+. There was significant increased in MDA level, indicating that GM-induced nephrotoxicity was mediated by oxidative stress reactions. Histopathological examination of renal tissues showed severe changes manifested by atrophy of glomerular tuft, necrosis of epithelial lining renal tubules with apoptosis of tubular epithelium and local renal hemorrhage. Simultaneous administration of ALE during GM therapy protected kidney tissues against its nephrotoxic effects as evidenced from amelioration of histopathological changes and normalization of kidney biochemical parameters. The most effective dose was 600 mg/kg b.wt/d as compared with the other two doses. ALE has nephroprotective and antioxidant effects and therefore it may be beneficial for patients with kidney diseases and who receiving GM therapy. |
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Supervisor |
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Prof.Dr. Hala Abd El-ERahman Hassan Khattb |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1436 AH
2015 AD |
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Co-Supervisor |
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Dr. Maha Ahmed Alahdab |
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Added Date |
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Thursday, October 8, 2015 |
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Researchers
| مها محمد عدنان وزان | Wazzan, Maha Adnan | Researcher | | |
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